Fig. 4

miR-3129 was expressed in the early stage of osteoblast differentiation and enhanced osteogenesis of hBMSCs. A Sequences and structure of human hsa-miR-3129, based on the miRBase database. B miR-3129 expression was examined at the indicated time points during osteogenesis of MSCs. The levels of miRNA expression were quantified by qPCR. The amounts of miRNA transcripts were expressed relative to the amount of U6sn transcript. Data are expressed as mean ± SD from three independent experiments. C Calcification was examined in MSCs and NHDF cells treated with Pre-miR-3129-5p and Pre-miR-NC by Alizarin Red S staining. Representative data are provided from three independent experiments (each n = 3 in B and C). *p<0.05, ***p<0.001 vs. day 1 by one-way ANOVA. N.S, not significant; RQ, relative quantification; OB, osteoblast. NHDF, normal human dermal fibroblast