Fig. 4

Par2KO improves survival from high-dose ConA-induced hepatitis. High-dose ConA (15mg/kg) induced acute liver lesions in all mice by 6 h after injection (A–C). Extensive hemorrhage was present in WT (B), but not in Par2KO (C) mice, 6 h after injection of ConA. Scale bar for A–C = 75μm. D, E T cell depletion rescued WT liver from damage—quantification of the data presented in supplemental fig 7. D Liver hemosiderin area was significantly greater in WT than in Par2KO mice 6 h after high-dose ConA injection (15 mg/kg, n=24, n=4 for each group). Following T cell depletion, there was no hemosiderin in the KO and almost none in the WT mice. E Hepatocyte necrosis appeared both in WT and Par2KO mice 6 h after high dose of ConA injection. T cell depletion diminished hepatocyte necrosis in the WT mice, while in the KO necrosis remained similar. Statistical significance was measured using T test. *Indicates p<0.05, **p<0.01, ***p<0.005, error bars = SEM. Black—between the WT groups; Red—in D, hemosiderin differences between WT and KO 6 h after ConA injection without T cell depletion. Green—in E, between the Par2KO groups. F Kaplan-Meier survival plot of WT (black, n=12) and Par2KO (green, n=12) after 15mg/kg ConA injection. Log-rank (Mantel-Cox) test for p value = 0.0004