Fig. 4From: Canonical Wnt signaling activation by chimeric antigen receptors for efficient cardiac differentiation from mouse embryonic stem cellsQuantitative gene expression analysis of mesodermal progenitor and differentiation markers in EBs derived from ES/scLRP6S/scFZD8L. A–D mRNA expression levels of mesodermal progenitor markers on day 6 (A), cardiac and non-cardiac mesodermal markers (B, C), and endodermal markers and ectodermal cell markers (D) on day 13. EBs were derived from ES/mock and ES/scLRP6S/scFZD8L stimulated with vehicle, 0.1 μg/ml BSA-FL, or 50 ng/ml Wnt3a from day 2 to day 3 of differentiation. The expression levels were normalized with those of an internal control (Gapdh). The data represent the mean and standard deviation from 3 to 6 independent experiments. *p < 0.05 (vehicle vs Wnt3a). †p < 0.05 (vehicle vs BSA-FL). ‡p < 0.05 (BSA-FL vs Wnt3a). Significance was assessed by Turkey’s multiple comparison testBack to article page