Fig. 1

Effects of aging on the formation and growth of the colonic epithelial organoids. a Number of organoids derived from young and old mice that were cultured at a density of 500 crypts per well in the primary organoid culture (left) and the average number in each group (right). Data are reported as the mean ± standard deviation (SD) of 30 wells (5 wells per mouse; 6 mice per group). b Representative images of young- and old-mouse organoids on day 5 of passage 0 after seeding. Scale bar represents 200 μm. c Changes in the areas of young- and old-mouse organoids at days 4 and 5. The represented percentage is relative to young-mouse organoids on day 4. Data are reported as the mean ± SD of 30 organoids (5 organoids per mouse; 6 mice per group). d The growth rate of young- and old-mouse organoids (5 organoids per mouse; 6 mice per group). Data are reported as the mean ± SD. e Representative images of young- and old-mouse organoids on day 5 of passages 1 and 2 (Scale bar = 200 μm). f Organoid formation of 500 crypts per well in the primary organoids on day 5 of passages 0, 1 and 2 (3 mice per group). Significance was calculated by the Mann–Whitney t-test. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001