Fig. 7
The formation and growth of colonic epithelial organoids were reduced in old mice with acute DSS-colitis. a Representative images of organoids on day 5 and 7 of passage 0 (organoids from young and old control and DSS-colitis mice sacrificed at 16 days after DSS initiation). b The number of organoids was cultured at a density of 2000 crypts per well. Data are reported as the mean ± standard deviation (SD) of 10 wells. c The relative organoid area from young and old DSS-colitis mice at days 6 and 7. The represented percentage is relative to the organoids of young DSS-colitis mice on day 6. Data are reported as the mean ± SD of 8 organoids. d The growth rate of the organoids from young and old DSS-colitis mice from day 6 to day 7. Data are reported as the mean ± SD of 8 organoids. e, f mRNA expression of TGF-β, Smad3, p16INK4a of the organoids from young and old control or DSS-colitis mice. Data are reported as the mean ± SD (n = 6). g Flow cytometric analysis of organoid cells (organoids from young and old control and DSS-colitis mice). Representative plots for Ly6a/Sca-1 positive cell population. Data are reported as the mean ± SD (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001,.****p < 0.0001