Fig. 2

RNA-Seq analysis of 661W and ARPE-19 cells treated with vehicle or STS. a The total number of upregulated or downregulated genes in four groups. b The top 20 most co-upregulated and co-downregulated genes and their average expression value in two types of cells. c The line chart of the CXCL2 gene expression showed that CXCL2 gene was upregulated in each biological replicate. d The quantitative PCR analyses of CXCL2 expression normalized to GAPDH in four groups. The data are presented as the means ± SEM (n = 4 or 5, *p < 0.05). e Morphological changes of 661W cells treated with 50 nM rCXCL2 or vehicle (medium) for 48 h. Scale bar, 20 μm. f β-III tubulin staining images of two-group 661W cells. Scale bar, 20 μm. g Representative neurite tracing images of (f). The average lengths of β-III tubulin-positive neurites were compared between the two groups. Data are shown as the means ± SEM, n = 6 samples per group, *p < 0.05