Fig. 3

rCXCL2 regenerates RGC axons in ONC models. a Timeline of injection process of rCXCL2/vehicle-treated groups within 7 or 14 days. b Anterograde CTB tracing of regenerated RGC axons from vehicle (PBS) or rCXCL2-treated eyes 7 and 14 days after ONC. Scale bar, 100 μm, *the crush site. c The black-and-white images of (b). d GAP43.⁺ regenerated axons after ONC with vehicle (PBS) or rCXCL2 injection. Scale bar, 100 μm. e The black-and-white images of (d). f Quantitation of CTB-positive axons 0.5 mm past the injury site. Data are presented as mean ± SEM, n = 4 to 5 nerves per group, *p < 0.05. g Quantitation of GAP43-positive axons 0.5 mm past the injury site. Results are presented as mean ± SEM, n = 4 to 5 nerves per group, *p < 0.05