Fig. 8

rCXCL2 binds with CXCR2 to activate different signaling pathways in ONC/NMDA-damaged retinas. a Immunofluorescence images of retinal sections to visualize the specific CXCL2 receptor, CXCR2 (green), RGCs (anti-RBPMS) (red), and cellular nucleus (DAPI) (blue). Scale bar, 20 μm. b Quantification of the number of CXCR2⁺ or RBPMS⁺ cells in each layer of retinal cells. Data are presented as mean ± SEM. c Western blots (cropped blot images) showing the protein expression of p-JAK2, p-STAT3, Bax, Bcl-2, caspase-3, and β-actin. d–h Western blot analysis of b, β-actin was used as an internal control. The results are shown as the means ± SEM (for c–f, n = 4; for g, n = 3, *p < 0.05, ns: not statistically significant). GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer