Fig. 2

In vitro mRNA transfection toward primary osteoblasts to induce osteogenic differentiation. A Western Blotting to confirm Runx2 or VEGF protein production after the mRNA transfection toward HeLa cells. B, C Relative mRNA expression levels of Osteopontin and Osteocalcin on days 3, 7, and 11 after the mRNA transfection. The levels were evaluated by RT-PCR. Results were normalized by Blank control on day 3. D Relative mRNA expression levels of β-catenin, Lef1, and Osx on days 7 after the mRNA transfection. Representative data were expressed as mean ± S.E.M. from three independent experiments, and analyzed by one-way ANOVA (post hoc test: Tukey’s multiple comparison test), n = 3/group. *:p < 0.05, **p < 0.001, compared with Blank group; ^#p < 0.05, ^##p < 0.001, compared with Luc2 group; ^δp < 0.05, ^δδp < 0.001, compared with other groups. E Time course of Gaussia Luciferase protein production after Gluc mRNA (1 μg) transfection toward primary osteoblasts