Fig. 3

In vivo mRNA administration into the bone defect using polyplex nanomicelles. A Luciferase expression visualized by IVIS imaging. 10 µg Luc2 mRNA was administered into the mandible bone defect. B Time course of Luciferase expression. The data of luciferase radiance were expressed as mean ± S.E.M., and analyzed by t-test, *:p < 0.05. C-G Immunohistological detection of ZsGreen1 expression 24 h after the administration of ZsGreen1 mRNA. C H&E staining (4 × objective, scale bar = 500 µm), D The serial section of (C) with immunohistological staining with anti-ZsGreen1 antibody. B: bone tissue. E–G Region of interests (ROIs) to detect ZsGreen1 signals. ROI-1: set for including the bone edge; ROI-2,3: set in the bone defect. (20 × objective, scale bar = 100 µm)