Fig. 4

Protein expressions from Runx2 mRNA or VEGF mRNA. A, B Pictures of the bone defect by H&E staining to define the area of ROIs. (4 × objective, scale bar = 500 µm) (C, D) Immunofluorescent staining with primary antibodies against anti-Runx2 (C) or anti-VEGF (D). The ROIs were set in the bone defect (20 × objective, scale bar = 100 µm)