Fig. 9

Immunohistological analysis using antibodies against ALP or OCN to evaluate osteoblastic differentiation. A, B Images stained by ALP antibody, with co-staining by DAPI (B) (20 × objective, scale bar = 100 µm). C, D Images stained by OCN antibody, with co-staining by DAPI (D). E, F Quantification of positive areas of ALP (E) or OCN (F), using Image J software. Data were expressed as mean ± S.E.M. and analyzed by one-way ANOVA (post hoc test: Tukey's multiple comparison test), n = 3/group. The symbols “*, **, ^#, ^##, ^δ, ^δδ” indicate the significant differences similarly as in Fig. 2