Table 1 Primer sequences for amplification of genes for RT- PCR
Genes | Symbol | Transcript No | Sequence (5′ → 3′) | Product (bp) |
|---|---|---|---|---|
β-actin | β-actin | NM_007393 | F: GTGACGTTGACATCCGTAAAGA R: GCCGGACTCATCGTACTCC | 245 |
Osteopontin | Spp1 | NM_001204201.1 | F: CCTGGCTGAATTCTGAGGGAC R: CTTCTGAGATGGGTCAGGCA | 185 |
Osteocalcin | Bglap | NM_007541.3 | F:GAACAGACAAGTCCCACACAGC R:TCAGCAGAGTGAGCAGAAAGAT | 79 |
β-catenin | Ctnnb1 | NM_007614.3 | F: TGGTGACAGGGAAGACATCA R: CCACAACAGGCAGTCCATAA | 112 |
Lef1 | Lef1 | NM_010703.4 | F: GATCCTGGGCAGAAGATGGC R: GCTGTCATTCTGGGACCTGT | 195 |
Osterix | Sp7 | NM_130458.4 | F: GATGGCGTCCTCTCTGCTTG R: GCGTATGGCTTCTTTGTGCC | 147 |