Fig. 1

Reduced AhR expression and its promoter activity after deletion of Jdp2. A Comparison of the levels of AhR protein in WT and Jdp2^–/– MEFs. B WT Jdp2 rescued AhR expression in Jdp2^–/– MEFs. WT Jdp2: wild-type Jdp2; 139Jdp2 (C139AJdp2): alanine-mutated Jdp2 at position 139 [44]. After selection with G418 and harvesting for testing, AhR expression was measured. C Comparison of AhR protein levels in WT and Jdp2^–/– MEFs treated with 10 nM TCDD for indicated time-periods. See Supplementary Figure S8 for the original full-length blot images. The intensity of each band was then quantified, and the relative value was normalized to β-actin. D Relative DRE promoter luciferase activity in WT and Jdp2^–/– MEFs in response to 10 nM TCDD for indicated time-periods. DRE luciferase activity in WT MEFs at 0 h was set at 1.0. Values represent the mean ± SEM (n = 3). Data were analyzed using one-way ANOVA with the Tukey post hoc test (*p < 0.05; **p < 0.01). E Relative ARE promoter luciferase activity in WT and Jdp2^–/– MEFs in response to 10 nM TCDD for indicated time-periods. ARE luciferase activity in WT MEFs at 0 h was set at 1.0. Values represent the mean ± SEM (n = 3). Data were analyzed using one-way ANOVA with the Tukey post hoc test (*p < 0.05; **p < 0.01). F Relative activity of pGL4.1-AhR luciferase in WT and Jdp2.^–/– MEFs treated with 10 nM TCDD for indicated time-periods. Luciferase activity was calculated as the ratio of the AhR luciferase activity to that of the control pGL4.1 and is expressed as relative luciferase activity. Values represent the mean ± SEM (n = 5). Data were analyzed using two-way ANOVA with the Bonferroni post hoc test (*p < 0.05; **p < 0.01)