Fig. 2

Characterization of the AhR promoter activity and transcription factors required for the full promoter. A Schematic representation of the positions of each DRE and ARE in the AhR promoter region. Each element is shown at the position from the putative transcription start sites, which were mutated to generate DRE and ARE mutants. B Effects of the mutation of each cis-element, ARE1, ARE2, DRE1, DRE2, and DRE3, on the AhR promoter region. Luciferase activity was measured in WT MEFs in the presence of 10 nM TCDD at indicated time-periods, as described in the “Methods” section. The luciferase activity of pGL4.1-AhR luciferase at 0, 2, 6, and 24 h was arbitrarily set at 1.0. Data were analyzed using one-way ANOVA with the Tukey post hoc test (*p < 0.05, n = 5). C, D Effects of siRNA against each representative transcription factor (AhR, Arnt, Nrf2, MafK, Jdp2, and Ahrr) on pGL4.1-AhR luciferase activity in WT MEFs in the absence (C) or presence (D) of 10 nM TCDD. The luciferase activity of pGL4.1 luciferase was arbitrarily set at 1.0. Data were analyzed using one-way ANOVA with the Tukey post hoc test (*p < 0.05; **p < 0.01, n = 3). E Effects of the dose of the Jdp2 expression vector (0, 25, 50, 100, or 200 ng) with 10 nM TCDD treatment in Jdp2.^–/– MEFs. DMSO was added at 0.1%. The expression levels of Jdp2 and β-actin are shown in cropped figures. See Supplementary Figure S8 for the original full-length blot images. The intensity of each band was then quantified, and the relative value was normalized to β-actin and is shown as a ratio. Data were analyzed using one-way ANOVA with the Tukey post hoc test (*p < 0.05, n = 3)