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Fig. 2 | Inflammation and Regeneration

Fig. 2

From: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo

Fig. 2

Expression of Rasip1 in Sox17 (GFP)-transduced CD45low c-Kithigh cells. A Upper panel: A schema of Sox17-IRES-GFP (Sox17) and IRES-GFP (Mock) retrovirus vectors. Lower panel: The red squares show a region of the CD45lowc-Kithigh cell population. The expression of Rasip1 in Mock- or Sox17-transduced cells was analyzed by RT-PCR and normalized by β-actin. B Upper panel: A schema of the Sox17-ERT-IRES-GFP retrovirus vector. Lower panel: The dark green squares show Sox17 (GFP)+ CD45lowc-Kit.high cells. After FACS sorting, expression analysis of the Rasip1 gene was performed in Sox17-ERT-IRES-GFP-transduced cells with or without tamoxifen (1.0 µg/ml)

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Inflammation and Regeneration

ISSN: 1880-8190

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