Fig. 5

Rasip1 overexpression (OE) in CD45^lowc-Kit^high cells. A A schema of IRES-GFP (Mock) and Rasip1-IRES-GFP retrovirus vectors. B Timeline of Rasip1 OE experiment. C Red squares show Mock- and Rasip1-transduced CD45^lowc-Kit^high cells. D After 4 days of Mock and Rasip1 transduction, CD45^lowc-Kit^high cells (2.5 × 10²) were embedded in a semisolid medium (colony assay 1). According to the timeline in B, CD45^lowc-Kit^high cells were sorted (5.0 × 10.²) after 8 days of Rasip1 transduction (Colony assay 2). The number of total (CFU-C) and multilineage (CFU-Mix) colonies was scored after 7 days of culture (n = 5). The pair of colony numbers (Mock and Rasip1) in each experimental group were plotted and connected by a line. Each symbol represents the colony numbers observed with each cell preparation from individual litters of E10.5 mice (n = 6, 6 individual litters of mice from which each experimental group was set up). *p ≤ 0.05