Fig. 3

Basic characterization of established iPSCs. See also Supplementary Fig. 2. A Representative phase contrast images of iPSC colonies. iPSC clones from the patients with (i) Duchenne muscular dystrophy, (ii) Alexander disease, (iii) Dravet syndrome and (iv) Smith-Magenis syndrome are shown. Scale bars = 100 μm. B Estimation of the cell type from which iPSCs originated; n = 1,532. C Estimation of the number of residual copies of episomal vectors remaining in iPSCs, calculated based on the quantitative values of EBNA and CAG promotors. Dashed lines are drawn at the line corresponding to one copy of remaining vector per cell; n = 1,532. D Correlation between OCT3/4 and NANOG expression in each clone. The single regression equation is plotted on the graph; n = 1,532. E, F Comparison of OCT3/4 (E) and NANOG (F) expression by sex (female n = 798, male n = 734). Values for each clone have been plotted relative to the expression levels in control 201B7 iPSCs. Statistical analysis was performed using the unpaired t-test (E) and Mann–Whitney U test (F)