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Fig. 2 | Inflammation and Regeneration

Fig. 2

From: A niche-mimicking polymer hydrogel-based approach to identify molecular targets for tackling human pancreatic cancer stem cells

Fig. 2

Optimization of the niche-mimicking functions of hit polymer PA531 by PEG-based hydrogelation. A The composition of monomers, cross-linkers, photoinitiators, NMP and water for the fabrication of five hydrogels. B Quantification of the mean fluorescence intensity (MFI) of ZsGreen and total cell number of KLM1-Gdeg cells cultured for 48 h on hydrogels. Data represent the averages ± standard deviation (SD) from three independent experiments. **P < 0.01, n.s: no significance. C Images of KLM1-Gdeg cells cultured on coverglass without hydrogel (HG-) and hit and control gels (HG4 and HG5). Scale bar = 200 μm. D Representative FACS plots of KLM1-Gdeg cells cultured on HG-, HG4, and HG5 for 5 days. This lot of hydrogel is different from that used for generating B. E The mean percentages ± SD of ZsGreen + cells are shown in plots and displayed in a bar graph. *P < 0.05

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Inflammation and Regeneration

ISSN: 1880-8190

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