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Fig. 3 | Inflammation and Regeneration

Fig. 3

From: Placenta mesenchymal stem cell-derived extracellular vesicles alleviate liver fibrosis by inactivating hepatic stellate cells through a miR-378c/SKP2 axis

Fig. 3

Characterization of Pd-MSCs and their extracellular vesicles. A Differentiation of MSCs displaying chondrogenesis, osteogenesis, and adipogenesis, which was validated by alcian blue, alizarin red, and oil red O staining, respectively. B Flow cytometric analyses of phenotypic markers of MSCs (positive markers, CD105, CD73, CD44, and CD90; negative markers, CD34, CD45, CD19, and HLA-DR). C Morphology of extracellular vesicles observed by transmission electron microscopy. D Size distribution of Pd-MSCs-extracellular vesicles measured by qNano analysis. E Exosome-specific markers CD9, CD63, CD81, and TSG101 were detected in conditioned medium of Pd-MSCs and exosome solution by western blotting. F Semi-quantitative analysis of protein expression in E. CM, conditioned medium; EVs, extracellular vesicles. Bar scale, 50 μm. *P < 0.05; **P < 0.01

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Inflammation and Regeneration

ISSN: 1880-8190

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