Fig. 4
Deficiency of CXCL10 prevents the increase of macrophages in the lung. A Representative immunostaining of lung macrophage using CD68 as a marker. Red arrows indicate CD68+ stained cells in lungs exposed to normoxia (NOX, 21% O2) or hyperoxia (HYX, 85% O2) at postnatal day 14 (P14). B Quantification of CD68+ cells per field of view. C-F Assessment of mRNA expression of Cxcr3 (C), Ccl2 (C–C Motif Chemokine Ligand 2) (D), Lgals3 (Galectin3) (E), and C1qb (complement C1q B chain) (F) in lungs at P14 after exposure to NOX or HYX. Mean ± SEM; n = 4–9/group. Two-way ANOVA: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns = not significant; scale bar: 40X