Fig. 6
CXCL10 deficiency limits the macrophage influx to the lung during the regeneration phase after postnatal hyperoxia. Experimental design: wildtype (WT) and Cxcl10 knockout (Cxcl10−/−) mice were exposed to normoxia (NOX, 21% O2) or hyperoxia (HYX, 85% O2) from birth until postnatal day 14 (P14); from P14 to P28, all mice were exposed to normoxia. A, B Representative immunostaining of macrophages using CD68 as a marker. Red arrows depict CD68+ stained cells (A). Quantification of CD68+ cells in the lung per field of view (B). C, D Measurement of mRNA expression of Cxcr3 (C), Ccl2 (C–C Motif Chemokine Ligand 2) (D), Lgals3 (Galectin3) (E), and C1qb (complement C1q B chain) (F) in lungs at P28; 18 s rRNA served as housekeeping gene. Mean ± SEM; n = 4–9/group; Unpaired t test: $p < 0.05; Two-way ANOVA: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; scale bar: 40X