Fig. 5

M1 macrophage secretome induces a myofibroblast-like secretory phenotype in ASCs. A, B Gene profiling by quantitative qPCR mRNA analysis of MQ-CM-treated ASCs at 72 h. Genes analyzed were clustered for A potential inflammatory, angiogenic, and keratinocyte (KC) mobilizing function (n = 3) or B ECM remodeling properties (n = 5). Data are shown as -ΔCt ± SEM. C Representative images of HUVEC-based in vitro angiogenesis assay and analysis thereof at 6 h. D Quantification of master meshes, master segments, branch lengths, and junctions of identified HUVEC structures. Data are shown as mean ± SEM (n = 6). Asterisks indicate p values of p<0.05 (*), p<0.01 (**), and p<0.001(***)