Fig. 2

Effect of chemotherapy on oxidative damage and mitochondrial dysfunction in the ovary. A Determination of antioxidant capacity and the level of MDA in serum and ovaries from the different treatment groups. B IHC staining was performed to examine the expression of DNA/RNA damage markers in ovaries. Scale bars, 50 μm and 25 μm. C–F The ultrastructure and mitochondrial morphology of antral follicles in the different groups were examined by TEM. Abnormal mitochondria were swollen with evidence of severely disrupted cristae, indicated by black arrows. Scale bars, 5 μm, 1 μm, 2 μm, and 500 nm. N=4 per group. The data were presented as the mean±SEM. *P<0.05, **P<0.01, and ***P<0.005. COC, cumulus-oocyte complex; GCs, granulosa cells; TCs, thecal cells; O-M, oocyte-mitochondrial; GCs-M, granulosa cell-mitochondrial; TCs-M, thecal cells-mitochondrial