Fig. 3

Effect of chemotherapy on cell death and inflammation-related fibrosis in the ovary. A Western blotting was used to examine the expression of apoptosis- and pyroptosis-related proteins in ovaries in the different treatment groups. B The columns showed that the expression of cleaved-CAS3, Bax/Bcl2, NLRP3, and cleaved-CAS1 significantly increased after chemotherapy. C ELISA was used to measure the level of inflammation-related cytokines in the serum of mice in the different treatment groups. D Representative images showing PSR-stained ovarian sections in the different groups. Scale bars, 100 μm and 25 μm. E The column displayed the area of fibrosis (PSR-positive staining) in ovaries in the different treatment groups. N=4 per group. The data were presented as the mean±SEM. *P<0.05, **P<0.01, and ***P<0.005