Fig. 4

Effect of TNF-α and IFN-γ costimulation on the characteristics of hAECs. A The table shows the number of differentially expressed cytokines in the conditioned medium of native hAECs (native-hAEC-CM) and activated hAECs (activated-hAEC-CM) from donors #1 and #2. B Venn diagram showing the overlap of upregulated differentially expressed cytokines between donors #1 and #2. C All differentially upregulated cytokines were listed in the Table. D GO analysis of the biological process, cellular component, and molecular function categories of the upregulated cytokines. E ELISA was used to verify the levels of important cytokines in native-hAEC-CM and activated-hAEC-CM. F Representative images of the tube formation assay results in native-hAEC-CM and activated-hAEC-CM treatment groups. G, H Quantitative analysis of tube formation was conducted by counting tubes and nodes. The data were presented as the mean±SEM. *P<0.05, **P<0.01, and ***P<0.005