Fig. 5

Effect of native hAECs and activated hAECs transplantation on injured ovaries. A, B Schematic diagrams showing the design of the animal experiment. C Representative images of ovaries in the different treatment groups. D The column showed the ovarian index (ovarian weight/body weight) of mice in the different treatment groups. E Native and activated hAECs were labeled with the fluorescent dye PKH26. Scale bar, 50 μm. F The red fluorescent signals in ovarian sections were observed under a fluorescence microscope. Scale bar, 50 μm. G The column showed the retention rate of transplanted native and activated hAECs in damaged ovaries. H The level of ROS in damaged ovaries was examined by immunofluorescent staining. Scale bar, 50 μm. I The column showed the level of ROS in ovaries after native and activated hAEC transplantation. J Double immunostaining of CD68 and CD163 in ovaries in the different groups. Scale bar, 10 μm. K Western blotting was performed to examine the expression of redox homeostasis-related proteins in ovaries. L, M The columns showed that activated hAEC transplantation significantly upregulated the expression of thioredoxin1/2 in damaged ovaries. N=4 per group. The data were presented as the mean±SEM. *P<0.05 and **P<0.01