Fig. 5

Improving neuronal vulnerability against excessive L-Glutamate treatment by co-culturing with astrocytes. a Representative immunocytochemistry images of the co-culture model and neuronal mono-culture with or without excessive L-Glutamate treatment (15 μg/mL) for one week stained with Calcein-AM reagent and an antibody specific for MAP2. Scale bar; 100 μm. b Quantification and comparison of surviving ratio of iPSC-derived neurons (Calcein-positive and MAP2-positive neurons/Hoechst) among the mono-culture model of neurons and the co-culture model with and without excessive L-Glutamate treatment (15 μg/mL) for one week (n = 50 each) (three independent experiments). Bars, mean ± SEM. ****p < 0.0001 (One-way ANOVA)