Fig. 7
Establishment of the co-culture model from the iPSCs of the familial AD donor with APP V717L mutation (APP2E26 line). a Genotyping results of the APP2E26 iPSCs showing the G > C point mutation at APP Exon 17 (APP V717L). b Representative immunocytochemistry images of the co-culture model derived from the APP2E26 iPSC line stained with antibodies specific for S100β, GFAP, and MAP2. Scale bar; 100 μm. c Relative Aβ1-42/Aβ1-40 ratio of the iPSC-derived neurons and co-culture model of control 201B7 line (neurons (n = 15); co-culture (n = 15)) and fAD APP2E26 line (neurons (n = 15); co-culture (n = 15)) (three independent experiments). Bars, mean ± SEM. ***p < 0.001; ****p < 0.0001 (Kruskal-Wallis test)