Fig. 8

Astrocytic phenotypes of AD (APP ^V717L mutation) developed in the co-culture model of iPSC-derived neurons and astrocytes. a Representative immunocytochemistry (S100β and GFAP) images of iPSC-derived astrocytes in the co-culture model derived from the control and familial AD iPSC lines. Scale bar; 50 μm. b Quantification and comparison of soma areas (μm²) among the control astrocytes (n = 15), APP ^V717L astrocytes (n = 15), control co-culture model (n = 30), and APP ^V717L co-culture model (n = 30) (three independent experiments). Bars, mean ± SEM. *p < 0.05 (Kruskal-Wallis test). c Representative immunocytochemistry images of the control (201B7 line) and familial AD (APP ^V717L line) iPSC-derived astrocytes in the co-culture stained with antibodies specific for S100β and GFAP. Scale bar; 100 μm. d Quantification of the GFAP/S100β ratio among the control astrocytes (n = 30), the APP V717L astrocytes (n = 30), control co-culture model (n = 30), and APP V717L co-culture model (n = 30) (three independent experiments). Bars, mean ± SEM. *p < 0.05; ** p < 0.01; ****p < 0.0001 (Kruskal-Wallis test). e Representative immunocytochemistry images of the co-culture model of control (201B7 line) iPSC-derived astrocytes with the control (201B7 line) or familial AD (the APP ^V717L line) iPSC-derived neurons. Scale bar; 100 μm. f Relative Aβ_1-42/Aβ_1-40 ratio of the co-culture models of control (201B7 line) iPSC-derived astrocytes with the control (201B7 line) (n = 20) or the familial AD (the APP ^V717L line) iPSC-derived neurons (n = 20). Bars, mean ± SEM. ****p < 0.0001 (Unpaired-t test). g Quantification of the GFAP/S100β ratio of the co-culture model of the control (201B7 line) iPSC-derived astrocytes with the control (201B7 line) (n = 30) or the familial AD (the APP ^V717L line) iPSC-derived neurons (n = 30) (three independent experiments). Bars, mean ± SEM. ***p = 0.0002 (Mann-Whitney test)