Fig. 1

Tβ15 can promote hair growth of mice both ex vivo and in vivo. a Simulation diagram of skin organ culture ex vivo. b The skin of C57 mice aged 6–8 weeks was cultured ex vivo for 7 days. c The representative images of skin cultured ex vivo for 7 days were stained by H&E. Scale bar = 50 μm. d Quantitative estimation of relative number of hair follicles in H&E stained images. Observed and counted the number of hair follicles in six different areas of the skin with a magnification of × 100 under an optical microscope (n = 3). e The nude mice transplanted with organoid organs in both armpits were observed and recorded for 12 days (n = 3). f From the injection of organs, the back hair of mice was measured and recorded every 3 days (n = 18). g H&E staining was performed on the skin of the organoid transplant site in mice; scale bar = 100 μm, 50 μm. h Quantitative estimation of hair follicle length and measurement with Image J (2.3.0) (n = 3). Values are expressed as mean ± SEM. Student’s t-test (*p < 0.05, **p < 0.005, ***p < 0.0005)