Fig. 3
From: Th22 is the effector cell of thymosin β15-induced hair regeneration in mice
The differentiation-promoting factor of Th22 can promote hair growth. a, b The representative images of skin cultured ex vivo for 7 days were stained by H&E. Scale bar =50 μm; quantitative estimation of hair follicle length and measurement with Image J (2.3.0) (n = 3). c, f PCNA (red arrow) and CCR10 (green arrow) were stained by immunofluorescence in skin cultured ex vivo for 7 days. The image in the lower right corner enlarges some hair follicles; quantify the fluorescence signal intensity of PCNA at 100 μm2 around the hair follicle (n = 10); quantify the number of CCR10 positive cells around each hair follicle (n = 10). In this process, we avoid selecting the hair shaft with yellow spontaneous fluorescence into the statistical area, that is, only the position of per 100 μm2 around the hair follicle is selected, and the expression of related proteins in this range is counted. Values are expressed as mean ± SEM. Student’s t-test (*p < 0.05, **p < 0.005, ***p < 0.0005)