Fig. 4From: Th22 is the effector cell of thymosin β15-induced hair regeneration in miceIL-22BP blocks Tβ15-induced hair growth in mice. a, b The representative images of skin cultured ex vivo for 7 days were stained by H&E. Scale bar = 50 μm; quantitative estimation of hair follicle length and measurement with Image J (2.3.0) (n = 3). c, f PCNA (red arrow) and CCR10 (green arrow) were stained by immunofluorescence in skin cultured ex vivo for 7 days. The image in the lower right corner enlarges some hair follicles; quantify the fluorescence signal intensity of PCNA at 100 μm2 around the hair follicle (n = 10); quantify the number of CCR10 positive cells around each hair follicle (n = 10). Values are expressed as mean ± SEM. Student’s t-test (*p < 0.05, **p < 0.005, ***p < 0.0005)Back to article page