Fig. 7

Evaluation of cell survival and tumor growth derived from neurite bundle transplantation. a Immunofluorescence images of membrane-bound GFP-labeled nerve organoids in the microfluidic device clearly visualized the green neurite bundles with anti-GFP antibody immunostaining. Scale bar = 300 μm. b Immunofluorescence images of neurite bundles labeled with membrane-bound GFP confirmed by anti-GFP antibody staining. Upper: Neurite bundles cultured with spheroids. Bottom: Neurite bundles cultured without spheroids for 2 weeks. Scale bars = 100 μm. c Immunofluorescence images of regenerated tissue of membrane-bound GFP-labeled nerve bundles at 1 and 2 weeks after implantation, stained with anti-GFP antibody. White and yellow arrowheads: neurite bundle human tissue was positively stained; Scale bar = 1 mm. d Magnified image of the regenerating nerve tissue with transplantation (yellow arrowhead). Scale bar = 100 μm. e Live cell tracking after implantation of ffLuc-labeled artificial nerves. No bioluminescence reactivity was observed at any time point from 0–12 after transplantation. Left: transplanted side. Right: healthy control side. f Quantitative analysis of the photon counts derived from the ffLuc-labeled transplanted tissue up to 12 weeks. g Immunofluorescence image of a sagittal section of regenerated tissue at 12 weeks after transplantation, and no fluorescence-positive areas were observed. Scale bar = 1 mm