Skip to main content
Fig. 1 | Inflammation and Regeneration

Fig. 1

From: Rapid and high-purity differentiation of human medium spiny neurons reveals LMNB1 hypofunction and subtype necessity in modeling Huntington’s disease

Fig. 1

The capacities of neural induction of proneural factors. A Typical proneural factors, including NGN2, ASCL1, NeuroD1, and miR-9/124, as well as the knockdown of PTBP1 by sgPTBP1, were respectively constructed into lentivirus vectors. B, C The capacities of neural induction of proneural factors were examined. NGN2 and ASCL1 contributed to over 90% changes into neural morphologies at 3 dpi, whereas miR-9/124 and sgPTBP1 barely induced changes. Scale bar, 50 μm. D The cell processes of induced cells were better, or at least faster, induced by NGN2, rather than by ASCL1. E Quantitative expression analysis of the induced cells. NGN2 mainly induced the generation of excitatory neurons (vGLUT1, vGLUT2, GRIN1, GRIA4 and GRIK1), whereas ASCL1 mainly induced the generation of inhibitory neurons (GAD1 and GAD2)

Back to article page

Inflammation and Regeneration

ISSN: 1880-8190

Contact us