Fig. 2

Characterization of induced neurons. A Four major neural subtypes including MSNs, GABA, MN, and DA were differentiated, in parallel, by induced expression of transcription factors in rtTA-expressing stable cells. B Neuron identities were examined by quantitative PCR of typical markers at 21 dpi. C, D Neuron identities were examined by immunostaining of typical markers at 21 dpi on the glia layer. Over 90% of TUJ1⁺ neurons were positive for MSN markers including DARPP32 and GAD2, and were devoid of other subtype markers such as vGLUT1, TH, and HB9. Scale bars, 20 μm. E Synapse formation was examined by immunostaining of SYN1. Scale bar, 150 μm. Inset scale bar, 50 μm. F Detection of MSN subtypes by quantitative PCR. MSN subtype markers were all expressed at higher levels in generated MSNs than those of pan-neurons