Fig. 5

The propensity of polyQ aggregations and NCT disturbance in HD-MSNs. A–C The polyQ aggregates were detected by immunostaining of HTT (MW8) and ubiquitin in differentiated neurons on the glia layer. The treatment with MG132 was applied to trigger the formation of aggregates (arrowheads). Scale bars, 10 μm and 20 μm. D The 2Gi2R reporter system containing 2xGFP::NES-2xRFP::NLS tracks protein translocation inside and outside the nucleus. E–G The NCT disturbance in differentiated neurons on the glia layer was assessed by the ratios of nucleic GFP (Nuc/Cyt GFP) and cytoplasmic RFP (Cyt/Nuc RFP), increasing of which indicates an impaired NCT. *p < 0.05; **p < 0.01; ***p < 0.001