Fig. 2

Increased TN-C in the early stage of BPD. A To establish the BPD model, newborn mice were exposed to 85% oxygen from postnatal day (P) 1 to P7. Chronic hyperoxic lung injury was evident in this BPD model, and the mice were housed under normoxic conditions. Flow chart showing the BPD model. B-D Lung morphometry was analyzed via H&E staining of tissue samples from surviving pups at P14. Scale bars = 100 μm. In the enlarged squares, scale bars = 10 μm. E–F TN-C expression in pulmonary tissues at P14 was measured by ELISA and Western blotting. G-H Representative sections of immunohistochemical analysis of TN-C from lungs developing in 21% oxygen or 85% oxygen at P14. Five animals/group were evaluated. Scale bars = 50 μm. The data are expressed as the means ± SEMs. *P < 0.05, ** P < 0.01, ***P < 0.001, **** P < 0.0001