Fig. 3

HIF-1α directly induces soluble TN-C expression in epithelial cells. A-B Soluble TN-C (sTN-C) was measured via Western blotting, and Ponceau S was used as a loading control to assess the total protein concentration in the cell culture supernatant. C HIF-1α protein expression in MLE-12 cells stimulated with 85% oxygen for 12 h. D-E TN-C protein expression in MLE-12 cells stimulated with the HIF-1α agonist DMOG under normoxia (D) or the HIF-1α inhibitor 2-MeOE2 under 85% hyperoxia (E) for 12 h was measured via Western blotting. The density quantification of TN-C or HIF-1α was expressed as a ratio relative to β-actin. *P < 0.05, ** P < 0.01, ***P < 0.001, **** P < 0.0001