Fig. 7

Excess TN-C slowed alveolar formation. A CCK-8 assay for determining the viability of MLE-12 cells incubated for 24 h under normoxia after low-dose sTN-C supplementation (8 ng/mL). B-C The migration ability of alveolar epithelial cells was measured by the scratch method. In a mouse model of BPD, newborn mice at P2, P4, P6, P8, and P10 received continual intraperitoneal injections of isotype rat IgG antibodies or TN-C neutralizing antibodies at a concentration of 1 μg/body. D-F Lung morphometry was analyzed via H&E staining of tissue samples from surviving pups at P14. Scale bar = 100 μm. G RAGE and SP-C protein expression in lung tissues. *P < 0.05, ** P < 0.01, ***P < 0.001, **** P < 0.0001