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Fig. 3 | Inflammation and Regeneration

Fig. 3

From: Effects of acute exposure to low-dose radiation on the characteristics of human bone marrow mesenchymal stromal/stem cells

Fig. 3

Characteristics of H-MSCs. BM-MSCs (lots A and B) were exposed to 4 Gy (H-MSCs) or 0.1 Gy (Ir-MSCs) γ-radiation, or were not exposed to γ-radiation (non-Ir-MSCs). (a, b) Expansion of lots A and B of H-MSCs (dashed lines) and non-Ir-MSCs (solid lines). The number of viable cells was counted weekly after γ-irradiation using 0.5% trypan blue. (c) Morphology of lots A and B of H-MSCs, Ir-MSCs, and non-Ir-MSCs. Representative phase contrast images are shown. Yellow arrows indicate H-MSCs. Bars, 20 μm. (d, e) Expression of adipogenic and osteogenic markers in lots A and B of H-MSCs (gray bars), Ir-MSCs (open bars), and non-Ir-MSCs (solid bars), as assessed by quantitative real-time PCR. Expression in H-MSCs and Ir-MSCs is shown relative to that in non-Ir-MSCs. Data are mean values ± SD (a, b, d, e). n = 4 per group (a, d) or n = 5 per group (b, e). *, p < 0.05; **, p < 0.01

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