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Fig. 1 | Inflammation and Regeneration

Fig. 1

From: Characterization of polydactyly-derived chondrocyte sheets versus adult chondrocyte sheets for articular cartilage repair

Fig. 1

Protocol for the fabrication of PD sheets and TKA sheets. a Fabrication of PD sheets. PDs obtained from polydactyly surgery were passaged once or twice and stocked at − 180 °C. After further expansion, P2 or P3 cells were seeded on temperature-responsive culture inserts and cultured for 2 weeks. b Fabrication of TKA sheets. Adult chondrocytes and synovial cells obtained from TKA surgery were stocked at P0 and P1, respectively. Chondrocytes were seeded on temperature-responsive culture inserts and cocultured with synovial cells for 2 weeks. Three chondrocyte sheets were layered and cultured for an additional week

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