Table 1 Theoretical number of chondrocyte sheets that can be fabricated from the collected cartilage tissue
|  | Wet weight of harvested cartilage tissue (g) | Cell count after enzymatic digestion (× 106) | Passage of cells used to fabricate sheets | Seeding cell density on temperature-responsive culture inserts | Number of layers | Theoretical number of sheets that can be fabricated |
|---|---|---|---|---|---|---|
PD sheets | 0.3 ± 0.2 | 0.17 ± 0.09 | 2 | 1 × 104 cells/cm2 | 1 | 693 |
3 | 3326 | |||||
TKA sheets | 9.2 ± 1.0 | 18.5 ± 6.5 | 0 | 5 × 104 cells/cm2 | 3 | 29 |