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Table 2 Assembly technology. The table shows several advantages and disadvantages of assembly technologies

From: Introduction of vasculature in engineered three-dimensional tissue

Technology

Vascularization engineered 3D tissues

Advantage

Disadvantage

References

(1) 3D scaffold-based technology

Self-organization within scaffold or recellularization native vasculature ECM within decellularized tissues

Controlled selforganization with scaffold characters or native ECM and shape

With exogenous ECM or animal experiments

[38]

(2) Cell sheet technology

Self-organization within layered cell sheets

Without exogenous scaffolds

Specific manipulation

[31]

(3) Nanofilm coating technology

Self-organization within laded cells

Without specific equipment

Manipulation of 3D tissues to transplantation

[29]

(4) 3D printer technology

Vascular shaping with 3D printing

Fabrication free artificial shape

Patterning size limitation and degradation for long cultivation

[37]