Fig. 4

Function of the hLYPD8 protein generated by a Pichia pastoris expression system. a ELISA assay for binding of soluble hLYPD8 protein from P. pastoris to the indicated species of intestinal bacteria. b, c Immunoblot analysis with anti-FLAG antibody for the supernatant and pellet of the mixture of soluble hLYPD8 protein and a gradient dose of flagella (b) or bacterial bodies (c) from P. mirabilis. d ELISA assay of LYPD8 binding to flagella of P. mirabilis. e, f Motility of P. mirabilis in semisolid agar with or without soluble hLYPD8 protein. Representative photos are shown (e). The radii of motility halos were measured at 4 h (f). Data are mean ± s.d. (n = 6 per group). g Motility of P. mirabilis in semisolid agar with a gradient concentration of soluble hLYPD8 protein. The radii of motility halos were measured at 4 h. Data are mean ± s.d. (n = 6 per group). h, i Motility of E. coli in semisolid agar with or without soluble hLYPD8 protein. Representative photos are shown (h). The radii of motility halos were measured at 4 h (i). Data are mean ± s.d. (n = 6 per group). *p < 0.05, **p < 0.01, ****p < 0.001. n.s. not significant