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Fig. 3 | Inflammation and Regeneration

Fig. 3

From: Amyloid β directly interacts with NLRP3 to initiate inflammasome activation: identification of an intrinsic NLRP3 ligand in a cell-free system

Fig. 3

IL-1β secretion from living cells.a Cell-based assay using human embryonic kidney (HEK) 293T cells transfected with NLRP3, ASC, caspase-1, and IL-1β. IL-1β concentrations were evaluated by ELISA after a 16-h incubation with Aβ monomers, oligomers, or fibrils. b Cell-based assay using human peripheral mononuclear cells. IL-1β concentrations were evaluated by ELISA after a 16-h incubation with Aβ oligomers. c Caspase-1 activation in cell lysates was detected by immunoblotting corresponding to b. Cleaved-caspase-1 is indicated by arrowheads

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Inflammation and Regeneration

ISSN: 1880-8190

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