Skip to main content
Fig. 2 | Inflammation and Regeneration

Fig. 2

From: Phospholipase A2 in skin biology: new insights from gene-manipulated mice and lipidomics

Fig. 2

Skin abnormalities in knockout and transgenic mice for various sPLA2s. a Developmental expression of sPLA2s in mouse skin as assessed by quantitative RT-PCR. Pla2g2f is expressed throughout the peri- to postnatal period, while the periodic pattern of Pla2g2e expression coincides with the hair cycle, which involves repeated cycles of growth (anagen; P0–15), regression (catagen; P15–20), rest (telogen; P20–25), and re-growth (the next anagen; beyond P25). A representative result of two independent experiments is shown. b Expression of several keratinocyte markers in Pla2g2f+/+ and Pla2g2f−/− keratinocytes cultured for the indicated periods with 1 mM Ca2+ (n = 4, mean ± SEM, *P < 0.05). Pla2g2f deficiency impairs the induction of S100a9 (an activation marker) and Krt1 (an SS marker), but not Krt14 (a SB marker), suggesting that sPLA2-IIF regulates keratinocyte differentiation and activation. c Microarray profiling (Agilent Technologies) of genes associated with hair follicles and epidermis in Pla2g2f-TG (IIF-TG) or PLA2G10-TG (X-TG) mice relative to WT mice. In both strains, similar sets of genes are decreased in hair follicles (green), which reflects alopecia, and increased in the epidermis (red), which reflects epidermal hyperplasia. d Hematoxylin and eosin staining of skins from WT and PLA2G10-TG mice at P25. Hair follicle distortion and epidermal thickening are evident in the TG mice. IRS, inner root sheath. All animal experiments were performed in accordance with the protocols approved by the Institutional Animal Care and Use Committees in accordance with the Japanese Guide for the Care and Use of Laboratory Animals

Back to article page