Fig. 3

Cyclic stretch inhibits ATP-stimulated IL-1β secretion in LPS-primed macrophages. The murine macrophage cell line J774.1 (a) and mouse bone marrow-derived macrophages (BMDM) (b) was primed with 100 ng/mL of E. coli LPS for 4 h followed by stimulation with 1 mM ATP for 2 h in the continuous presence of LPS. Cells were exposed to cyclic stretch of 20% elongation at a frequency of 10 cycles/min for the first 2 h after the addition of LPS. Significance is indicated (*P < 0.05 significantly different from the positive control). CS, cyclic stretch. ND, not detected