Fig. 4

Cyclic stretch does not alter the LPS-induced NF-κB signaling pathway. a J774.1 cells were exposed to cyclic stretch of 20% elongation at a frequency of 10 cycles/min with 100 ng/mL LPS for the indicated times. Cell lysates were analyzed by western blotting with anti-IκB-α. An antibody against β-actin was used as a control. b J774.1 cells were exposed to cyclic stretch of 20% elongation at a frequency of 10 cycles/min for the first 2 h during treatment with 100 ng/mL LPS for 4 h. Nuclear proteins were extracted from cells and an NF-κB ELISA assay was performed. CS, cyclic stretch. ns, not significant