Fig. 2
The impact of γδ-selection on αβT/γδT cell fate decision. a Scheme of experimental procedure. γδT cells from thymus of Lat-deficient mice were sorted by FACS, infected with retroviruses expressing Lat along with EGFP, and cultured on Tst4/Dll4 stromal cells [103] for 5 days. b Expression of TCRδ chain and CD5 in the cultured cells with or without Lat. c EGFP+ (Lat-transduced) cells and EGFP− (non-transduced) were subjected to qRT-PCR to measure the mRNA expression levels of indicated genes. The heat-map indicates the relative gene expression